Update file mycoplasma.md

New Mycoplasma contamination check card

external/lab-good-practice/mycoplasma/mycoplasma.md

@@ -23,17 +23,28 @@ Characteristics of mycoplasma:
 - they lack a cell wall
 - they are fastidious in their growth requirements
 
-The problem with mycoplasma is that they have the ability to alter their host culture's cell function, growth, metabolism, morphology, attachment, membranes, virus propagation and yield, interferon induction and yield, cause chromosomal aberrations and damage, and cytopathic effect including plaque formation. 
-
 
+Mycoplasma have the ability to alter their host culture's cell function, growth, metabolism, morphology, attachment, membranes, virus propagation and yield, interferon induction and yield, cause chromosomal aberrations and damage, and cytopathic effect including plaque formation. 
 
+These 3 simple characterists, combined with their ability to alter virtually every cellular function and parameter, make mycoplasma the most serious, widespread, and devastating culture contaminants. 
 
 
 It is therefore crucial to regularly check for mycoplasma contamination.
 
+### Sources of mycoplasma contamination
+
+The number one source of mycoplasma contamination is other infected cell lines. Cell lines cultured in for example the same hood can easily become infected if there is a contamination in a single one.
+
+The second common source is the cell culturist; good training especially in aseptic techniques combined with the strategic use of a tested cell repository will greatly reduce the opportunities for contamination via this route. Talking and sneezing can generate significant amounts of aerosols that have been shown to contain mycoplasma. Also diry lab coats can be a source of contamination when a dust-laden sleeve is put into a laminar flow hood and dust particles fall into cultures.
+
+Contaminated sera and other filtration products have been in the past a major source of contamination, but nowadays they only play a minor role in mycoplasma contaminations.
+
+
+## Mycoplasma detection
+
 
 
-## Mycoplasma Control Kits
+### Mycoplasma Control Kits
 
 There are several methods for checking whether you have mycoplasma contaminations. 
 They include .....