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# comparison-protocols
# comparison-protocols
This project corresponds to the paper: A comparison of two iPSC differentiation protocols to generate human astrocyte cultures (Mulica et al)
This project corresponds to the paper: "A comparison of two iPSC differentiation protocols to generate human astrocyte cultures" (Mulica et al.)
#Figure2
## Folder `Figure2`
In this folder the codes for analyzing astrocytic morphology are stored, both for Oksanen and Palm astrocytes.
Subfolder 'oksanen-astrocytes' contains the Matlab codes for analyzing the morphology of Oksanen astrocytes. The Matlab version: 2020a. The corresponding raw data is stored at:
In this folder, the codes for analyzing astrocytic morphology are stored, both for Oksanen and Palm astrocytes.
/mnt/AtlasHCS/YokogawaCV8000Standalone/CellPathfinder/Patrycja/PKM_20210927_gfap_astro_OksanenMorph_20210927_205648/AssayPlate_PerkinElmer_CellCarrierUltra96
 
Subfolder `oksanen-astrocytes` contains the Matlab codes for analyzing the morphology of Oksanen astrocytes. The Matlab version used was: 2020a.
 
The corresponding raw data is stored at:
 
```
 
/mnt/AtlasHCS/YokogawaCV8000Standalone/CellPathfinder/Patrycja/PKM_20210927_gfap_astro_OksanenMorph_20210927_205648/AssayPlate_PerkinElmer_CellCarrierUltra96
/mnt/AtlasHCS/YokogawaCV8000Standalone/CellPathfinder/Patrycja/PKM_20210927_gfap_astro2_OksanenMorph2_20210927_222659/AssayPlate_PerkinElmer_CellCarrierUltra96
/mnt/AtlasHCS/YokogawaCV8000Standalone/CellPathfinder/Patrycja/PKM_20210927_gfap_astro2_OksanenMorph2_20210927_222659/AssayPlate_PerkinElmer_CellCarrierUltra96
 
```
Subfolder 'palm-astrocytes'contains the Matlab codes for analyzing the morphology of Palm astrocytes. The Matlab version: 2020a. The corresponding raw data is stored at:
Subfolder `palm-astrocytes` contains the Matlab codes for analyzing the morphology of Palm astrocytes. The Matlab version used was: 2020a. The corresponding raw data is stored at:
 
```
/mnt/AtlasHCS/YokogawaCV8000Standalone/CellPathfinder/Patrycja/PKM_20210927_gfap_astro_PalmMorph_20211028_152159/AssayPlate_PerkinElmer_CellCarrierUltra96
/mnt/AtlasHCS/YokogawaCV8000Standalone/CellPathfinder/Patrycja/PKM_20210927_gfap_astro_PalmMorph_20211028_152159/AssayPlate_PerkinElmer_CellCarrierUltra96
 
```
Subfolder 'r-scripts' contain R scripts used to summarize the data. The graphs were generated using the summarized data in GraphPad.
Subfolder `r-scripts` contain R scripts used to summarize the data. The graphs were generated using the summarized data in GraphPad.
#Figure3, 4A, B5
# Folder `Figure3, 4A, B5`
The analysis was performed in R.
The analysis was performed in R.
Raw data is stored: bds-02.uni.lux server in the "/mnt/data"
Raw data is stored at `bds-02.uni.lux` server in directory `/mnt/data`
 
 
# Folder `Figure 4C, D, E and S3`
#Figure 4C, D, E and S3
The analysis was performed in R.
The analysis was performed in R.
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