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the expression of M protein prevents the formation of TRAF3·TANK·TBK1/IKKϵ complex and thereby inhibits TBK1/IKKϵ-dependent activation of IRF3/IRF7 transcription factors. (HEK293/TLR3 stable cell line)
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Infection of macrophages with N15m1, which expresses an unstable nsp15, or N15m3, which expresses a catalysis-deficient nsp15, activated MDA5, PKR, and the OAS/RNase L system, resulting in an early, robust induction of type I IFN, PKR-mediated apoptosis, and RNA degradation.
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</p>
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</p>
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</rdf:RDF></annotation></qual:transition><qual:transition qual:id="tr_sa475"><qual:listOfInputs><qual:input qual:qualitativeSpecies="csa102" qual:transitionEffect="none" qual:sign="positive" qual:id="tr_sa475_in_0" /><qual:input qual:qualitativeSpecies="sa352" qual:transitionEffect="none" qual:sign="positive" qual:id="tr_sa475_in_1" /></qual:listOfInputs><qual:listOfOutputs><qual:output qual:qualitativeSpecies="sa475" qual:transitionEffect="assignmentLevel" qual:id="tr_sa475_out" /></qual:listOfOutputs><qual:listOfFunctionTerms><qual:defaultTerm qual:resultLevel="0" /><qual:functionTerm qual:resultLevel="1"><math xmlns="http://www.w3.org/1998/Math/MathML"><apply><and /><apply><eq /><ci>csa102</ci><cn type="integer">1</cn></apply><apply><eq /><ci>sa352</ci><cn type="integer">1</cn></apply></apply></math></qual:functionTerm></qual:listOfFunctionTerms><notes><html xmlns="http://www.w3.org/1999/xhtml"><head><title /></head><body><p>"Our results showed that the SARS-CoV N protein can significantly activate NF-kappaB only in Vero E6 cells, which are susceptible to SARS-CoV infection, but not in Vero or HeLa cells. This suggests that NF-kappaB activation is cell-specific."
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