Verified Commit 19e30577 authored by Laurent Heirendt's avatar Laurent Heirendt
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update title and abstract

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{% rtitle Protein Relative Abundance Quantification Algorithm for 3D Fluorescent Images from Tissue %}
{% rtitle PaFSe: a Parameter Free Segmentation Approach for 3D Fluorescent Images %}
Corrado Ameli, Sonja Fixemer, David Bouvier, Alexander Skupin.
In confocal fluorescent microscopy, the quality of the acquisition depends on several factors including the microscope parameterization, the light exposure time, the type and concentration of the antibodies used, the thickness of the sample and the tissue degradation. Due to these factors, intra-sample and inter-sample variability inevitably arises. Protein segmentation and quantification can thus become a challenging process. Therefore, image processing techniques need to address the acquisitions variability to minimize the risk of bias coming from changes in signal intensity, background noise and parameterization.
Confocal fluorescent microscopy is a major tool to investigate the molecular orchestration of biomedical samples. The quality of the image acquisition depends critically on the tissue quality and thickness, the type and concentration of antibodies used as well as on microscope parameters. Due to these factors, intra-sample and inter-sample variability inevitably arises. Segmentation and quantification of targeted proteins can thus become a challenging process. Image processing techniques need therefore to address the acquisitions variability to minimize the risk of biases originating from changes in signal intensity, background noise and parameterization.Here, we introduce PaFSe, a parameter free segmentation algorithm for 3D fluorescent images. The algorithm is based on our established PRAQA approach, which evaluates the dispersion of several pixel intensity neighborhoods allowing for a statistical assessment whether individual subfields of an image can be considered as positive signal or background. PaFSe extends PRAQA by a fully automatic estimate for the segmentation parameters and thereby provides a completely parameter free and robust segmentation algorithm. By comparing PaFSe with Ilastik on synthetic examples, we show that our method achieves similar performances as a supervised approach in low to moderate noise environments without the need of tedious training. Furthermore, we validate the efficiency of PaFSe by segmenting and quantifying the abundance of hyperphosphorylated Tau protein in post-mortem human brain samples from Alzheimer's disease patients and age-matched controls, where we obtain quantification values highly correlated with manual neuropathological segmentation.PaFSe is a parameter free, fast and adaptive approach for robust segmentation and quantification of protein abundance from complex 3D fluorescent images.
permalink: [doi:10.17881/j20h-pa27](https://doi.org/10.17881/j20h-pa27)
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