/***************************************************************************** multiBamCovMain.cpp (c) 2009 - Aaron Quinlan Hall Laboratory Department of Biochemistry and Molecular Genetics University of Virginia aaronquinlan@gmail.com Licenced under the GNU General Public License 2.0 license. ******************************************************************************/ #include "multiBamCov.h" #include "version.h" using namespace std; // define our program name #define PROGRAM_NAME "multiBamCov" // define our parameter checking macro #define PARAMETER_CHECK(param, paramLen, actualLen) (strncmp(argv[i], param, min(actualLen, paramLen))== 0) && (actualLen == paramLen) // function declarations void ShowHelp(void); int main(int argc, char* argv[]) { // our configuration variables bool showHelp = false; // input files string bedFile; vector<string> bamFiles; int minQual = 0; // input arguments bool haveBed = false; bool haveBams = false; bool properOnly = false; bool keepDuplicates = false; bool keepFailedQC = false; // check to see if we should print out some help if(argc <= 1) showHelp = true; for(int i = 1; i < argc; i++) { int parameterLength = (int)strlen(argv[i]); if((PARAMETER_CHECK("-h", 2, parameterLength)) || (PARAMETER_CHECK("--help", 5, parameterLength))) { showHelp = true; } } if(showHelp) ShowHelp(); // do some parsing (all of these parameters require 2 strings) for(int i = 1; i < argc; i++) { int parameterLength = (int)strlen(argv[i]); if(PARAMETER_CHECK("-bed", 4, parameterLength)) { if ((i+1) < argc) { haveBed = true; bedFile = argv[i + 1]; i++; } } else if(PARAMETER_CHECK("-bams", 5, parameterLength)) { if ((i+1) < argc) { haveBams = true; i = i+1; string file = argv[i]; while (file[0] != '-' && i < argc) { bamFiles.push_back(file); i++; if (i < argc) file = argv[i]; } i--; } } else if(PARAMETER_CHECK("-q", 2, parameterLength)) { if ((i+1) < argc) { minQual = atoi(argv[i + 1]); i++; } } else if(PARAMETER_CHECK("-p", 2, parameterLength)) { properOnly = true; } else if(PARAMETER_CHECK("-D", 2, parameterLength)) { keepDuplicates = true; } else if(PARAMETER_CHECK("-F", 2, parameterLength)) { keepFailedQC = true; } else { cerr << endl << "*****ERROR: Unrecognized parameter: " << argv[i] << " *****" << endl << endl; showHelp = true; } } if (!showHelp) { MultiCovBam *mc = new MultiCovBam(bamFiles, bedFile, minQual, properOnly, keepDuplicates, keepFailedQC); mc->CollectCoverage(); delete mc; return 0; } else { ShowHelp(); } } void ShowHelp(void) { cerr << endl << "Program: " << PROGRAM_NAME << " (v" << VERSION << ")" << endl; cerr << "Author: Aaron Quinlan (aaronquinlan@gmail.com)" << endl; cerr << "Summary: Counts sequence coverage for multiple bams at specific loci." << endl << endl; cerr << "Usage: " << PROGRAM_NAME << " [OPTIONS] -bams aln.1.bam aln.2.bam ... aln.n.bam -bed <bed/gff/vcf>" << endl << endl; cerr << "Options: " << endl; cerr << "\t-bams\t" << "The bam files." << endl << endl; cerr << "\t-bed\t" << "The bed file." << endl << endl; cerr << "\t-q\t" << "Minimum mapping quality allowed. Default is 0." << endl << endl; cerr << "\t-D\t" << "Include duplicate-marked reads. Default is to count non-duplicates only" << endl << endl; cerr << "\t-F\t" << "Include failed-QC reads. Default is to count pass-QC reads only" << endl << endl; cerr << "\t-p\t" << "Only count proper pairs. Default is to count all alignments with MAPQ" << endl; cerr << "\t\t" << "greater than the -q argument, regardless of the BAM FLAG field." << endl << endl; // end the program here exit(1); }