From 046dbde5544f2de06bd6cfa34ac611d8f57b2bf1 Mon Sep 17 00:00:00 2001 From: Aaron <aaronquinlan@gmail.com> Date: Sun, 3 Jan 2010 13:26:45 -0500 Subject: [PATCH] Generalized binKeeperFind to FindOverlapsPerBin --- bin/coverageBed | Bin 90480 -> 90544 bytes bin/windowBed | Bin 94968 -> 94864 bytes obj/bedFile.o | Bin 77792 -> 78672 bytes obj/coverageBed.o | Bin 30500 -> 16872 bytes obj/coverageMain.o | Bin 6900 -> 7356 bytes obj/lineFileUtilities.o | Bin 5576 -> 5568 bytes obj/windowBed.o | Bin 28772 -> 17200 bytes obj/windowMain.o | Bin 12588 -> 12360 bytes src/closestBed/b.bed | 4 +- src/closestBed/closestBed.cpp | 63 +- src/coverageBed/a.bed | 8 +- src/coverageBed/b.bed | 6 +- src/coverageBed/coverageBed.cpp | 80 +- src/coverageBed/coverageBed.h | 2 + src/coverageBed/coverageMain.cpp | 5 + src/coverageBed/new | 0 src/intersectBed/chr21.genes.bed | 828 ++++++++++++++++++ src/intersectBed/chr21.genes.txt | 828 ++++++++++++++++++ src/intersectBed/intersectBed.cpp | 100 +-- src/pairToBed/pairToBed.cpp | 6 +- src/pairToPair/pairToPair.cpp | 8 +- src/shuffleBed/shuffleBed.cpp | 2 +- src/subtractBed/new | 1 + src/subtractBed/old | 1 + src/subtractBed/subtractBed.cpp | 20 +- src/utils/bedFile/bedFile.cpp | 194 ++-- src/utils/bedFile/bedFile.h | 105 ++- .../lineFileUtilities/lineFileUtilities.cpp | 3 +- .../lineFileUtilities/lineFileUtilities.h | 2 +- src/windowBed/new | 4 + src/windowBed/old | 0 src/windowBed/windowBed.cpp | 56 +- src/windowBed/windowMain.cpp | 1 - 33 files changed, 1988 insertions(+), 339 deletions(-) create mode 100644 src/coverageBed/new create mode 100644 src/intersectBed/chr21.genes.bed create mode 100644 src/intersectBed/chr21.genes.txt create mode 100644 src/subtractBed/new create mode 100644 src/subtractBed/old create mode 100644 src/windowBed/new create mode 100644 src/windowBed/old diff --git a/bin/coverageBed b/bin/coverageBed index 3f9086fe300f9409f985f2c0b89f7e01561c096d..6ee16e6ef711715bda3a8f81732120d8247f5115 100755 GIT binary 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z)wHNK!=XhrEvjizO^a$;RMVoG7B#e}p+yZXYRn$cqJ|bVw5Xv)4J~SDQA>+jTGY~_ zmKL?NsHH_MEox~|ON&}sETzR#r&_;uZO-TLVWU#HJ=O>4&S{UxM8j&ytbyS<%Krd& C|Gb?5 diff --git a/src/closestBed/b.bed b/src/closestBed/b.bed index 807c0cd0..9b0e3730 100644 --- a/src/closestBed/b.bed +++ b/src/closestBed/b.bed @@ -1,3 +1,5 @@ chr1 20000 30000 geneA 100 + chr1 20000 30000 geneB 100 - - +chr1 19999 30000 geneC 100 + +chr1 80 90 geneD 100 + +chr1 112 1000 geneE 100 + diff --git a/src/closestBed/closestBed.cpp b/src/closestBed/closestBed.cpp index e3d0bb69..07aeaa87 100755 --- a/src/closestBed/closestBed.cpp +++ b/src/closestBed/closestBed.cpp @@ -83,51 +83,39 @@ void BedClosest::FindWindowOverlaps(BED &a, vector<BED> &hits) { while ((numOverlaps == 0) && (slop <= MAXSLOP)) { - if ((a.start - slop) > 0) { - aFudgeStart = a.start - slop; - } - else { - aFudgeStart = 0; - } - if ((a.start + slop) < 2 * MAXSLOP) { - aFudgeEnd = a.end + slop; - } - else { - aFudgeEnd = 2 * MAXSLOP; - } + // add some slop (starting at 0 bases) to a in hopes + // of finding a hit in B + if ((a.start - slop) > 0) aFudgeStart = a.start - slop; + else aFudgeStart = 0; + + if ((a.start + slop) < 2 * MAXSLOP) aFudgeEnd = a.end + slop; + else aFudgeEnd = 2 * MAXSLOP; - bedB->binKeeperFind(bedB->bedMap[a.chrom], aFudgeStart, aFudgeEnd, hits); + bedB->FindOverlapsPerBin(a.chrom, aFudgeStart, aFudgeEnd, a.strand, hits, this->forceStrand); - for (vector<BED>::iterator h = hits.begin(); h != hits.end(); ++h) { - - // if forcing strandedness, move on if the hit - // is not on the same strand as A. - if ((this->forceStrand) && (a.strand != h->strand)) { - continue; // continue force the next iteration of the for loop. - } - + vector<BED>::const_iterator h = hits.begin(); + vector<BED>::const_iterator hitsEnd = hits.end(); + for (; h != hitsEnd; ++h) { + numOverlaps++; // do the actual features overlap? int s = max(a.start, h->start); int e = min(a.end, h->end); - + int overlapBases = (e - s); // the number of overlapping bases b/w a and b + int aLength = (a.end - a.start); // the length of a in b.p. + if (s < e) { - // is there enough overlap (default ~ 1bp) - float overlap = (float)(e-s) / (float)(a.end - a.start); - - if ( overlap > 0 ) { - + float overlap = (float) overlapBases / (float) aLength; + if ( overlap > 0 ) { // is this hit the closest? if (overlap > maxOverlap) { closestB.clear(); closestB.push_back(*h); maxOverlap = overlap; } - else if (overlap == maxOverlap) { - closestB.push_back(*h); - } + else if (overlap == maxOverlap) closestB.push_back(*h); } } else if (h->end < a.start){ @@ -136,9 +124,7 @@ void BedClosest::FindWindowOverlaps(BED &a, vector<BED> &hits) { closestB.push_back(*h); minDistance = a.start - h->end; } - else if ((a.start - h->end) == minDistance) { - closestB.push_back(*h); - } + else if ((a.start - h->end) == minDistance) closestB.push_back(*h); } else { if ((h->start - a.end) < minDistance) { @@ -146,16 +132,11 @@ void BedClosest::FindWindowOverlaps(BED &a, vector<BED> &hits) { closestB.push_back(*h); minDistance = h->start - a.end; } - else if ((h->start - a.end) == minDistance) { - closestB.push_back(*h); - } + else if ((h->start - a.end) == minDistance) closestB.push_back(*h); } - } - /* if no overlaps were found, we'll - widen the range by SLOPGROWTH in each direction - and search again. - */ + // if no overlaps were found, we'll widen the range + // by SLOPGROWTH in each direction and search again. slop += SLOPGROWTH; } } diff --git a/src/coverageBed/a.bed b/src/coverageBed/a.bed index 85f30535..0f5d644c 100644 --- a/src/coverageBed/a.bed +++ b/src/coverageBed/a.bed @@ -1,5 +1,5 @@ -chr1 10 20 -chr1 20 30 -chr1 30 40 -chr1 100 200 +chr1 10 20 a1 1 - +chr1 20 30 a2 1 - +chr1 30 40 a3 1 - +chr1 100 200 a4 1 + diff --git a/src/coverageBed/b.bed b/src/coverageBed/b.bed index 771446e5..d881601d 100644 --- a/src/coverageBed/b.bed +++ b/src/coverageBed/b.bed @@ -1,3 +1,3 @@ -chr1 0 100 -chr1 100 200 -chr2 0 100 +chr1 0 100 b1 1 + +chr1 100 200 b2 1 - +chr2 0 100 b3 1 + diff --git a/src/coverageBed/coverageBed.cpp b/src/coverageBed/coverageBed.cpp index 388122e4..d7d6f949 100755 --- a/src/coverageBed/coverageBed.cpp +++ b/src/coverageBed/coverageBed.cpp @@ -40,7 +40,6 @@ void BedCoverage::GetCoverage(istream &bedInput) { string bedLine; int lineNum = 0; // current input line number vector<string> bedFields; // vector for a BED entry - bedFields.reserve(12); // process each entry in A @@ -49,55 +48,76 @@ void BedCoverage::GetCoverage(istream &bedInput) { lineNum++; Tokenize(bedLine,bedFields); BED a; - - // find the overlaps with B if it's a valid BED entry. - if (bedA->parseLine(a, bedFields, lineNum)) { - - // increment the count of overlaps for each feature in B that - // overlaps the current A interval - bedB->countHits(bedB->bedMap[a.chrom], a, this->forceStrand); + + if (bedA->parseLine(a, bedFields, lineNum)) { + // count a as a hit with all the relevant features in B + bedB->countHits(a, this->forceStrand); } // reset for the next input line bedFields.clear(); } - // now, report the count of hist for each feature in B. - for (masterBedMap::iterator c = bedB->bedMap.begin(); c != bedB->bedMap.end(); ++c) { - map<int, vector<BED> > bin2Beds = c->second; - - for (map<int, vector<BED> >::iterator b = bin2Beds.begin(); b != bin2Beds.end(); ++b) { - - vector<BED> beds = b->second; - for (unsigned int i = 0; i < beds.size(); i++) { - + //vector<int> depths; // track the discrete depths for each base in B + // used to calculate, min, max, median, etc. + + // now, report the count of hits for each feature in B. + masterBedMap::const_iterator chromItr = bedB->bedMap.begin(); + masterBedMap::const_iterator chromEnd = bedB->bedMap.end(); + for (; chromItr != chromEnd; ++chromItr) { + + binsToBeds::const_iterator binItr = chromItr->second.begin(); + binsToBeds::const_iterator binEnd = chromItr->second.end(); + for (; binItr != binEnd; ++binItr) { + + vector<BED>::const_iterator bedItr = binItr->second.begin(); + vector<BED>::const_iterator bedEnd = binItr->second.end(); + for (; bedItr != bedEnd; ++bedItr) { + int zeroDepthCount = 0; int depth = 0; + int start = min(bedItr->minOverlapStart, bedItr->start); - int start = min(beds[i].minOverlapStart, beds[i].start); - - for (int pos = start+1; pos <= beds[i].end; pos++) { - - if (beds[i].depthMap.find(pos) != beds[i].depthMap.end()) { - depth += beds[i].depthMap[pos].starts; + for (int pos = start+1; pos <= bedItr->end; pos++) { + + if (bedItr->depthMap.find(pos) != bedItr->depthMap.end()) { + + map<unsigned int, DEPTH> dMap = bedItr->depthMap; + depth += dMap[pos].starts; + //depths.push_back(depth); - if ((depth == 0) && (pos > beds[i].start) && (pos <= beds[i].end)) { + if ((depth == 0) && (pos > bedItr->start) && (pos <= bedItr->end)) { zeroDepthCount++; } - depth = depth - beds[i].depthMap[pos].ends; + depth = depth - dMap[pos].ends; } else { - if ((depth == 0) && (pos > beds[i].start) && (pos <= beds[i].end)) { + if ((depth == 0) && (pos > bedItr->start) && (pos <= bedItr->end)) { zeroDepthCount++; } + //depths.push_back(depth); } } // Report the coverage for the current interval. - int length = beds[i].end - beds[i].start; - - bedB->reportBedTab(beds[i]); - printf("%d\t%d\t%d\t%0.7f\n", beds[i].count, (length-zeroDepthCount), length, (float) (length-zeroDepthCount)/length); + int length = bedItr->end - bedItr->start; + int nonZeroBases = (length-zeroDepthCount); + float fractCovered = (float) nonZeroBases /length; + //sort(depths.begin(), depths.end()); + + bedB->reportBedTab(*bedItr); + printf("%d\t%d\t%d\t%0.7f\n", bedItr->count, nonZeroBases, length, fractCovered); + /* + cout << bedItr->count << "\t"; + cout << (length-zeroDepthCount) << "\t"; + cout << length << "\t"; + cout << setw (10); + cout << fractCovered << "\t"; + cout << *min_element(depths.begin(), depths.end()) << "\t"; + cout << *max_element(depths.begin(), depths.end()) << "\t"; + cout << *(depths.begin()+depths.size()/2) << "\n"; + depths.clear(); + */ } } } diff --git a/src/coverageBed/coverageBed.h b/src/coverageBed/coverageBed.h index e4654e64..25d340a0 100755 --- a/src/coverageBed/coverageBed.h +++ b/src/coverageBed/coverageBed.h @@ -14,7 +14,9 @@ #include "bedFile.h" #include <vector> +#include <algorithm> #include <iostream> +#include <iomanip> #include <fstream> #include <stdlib.h> diff --git a/src/coverageBed/coverageMain.cpp b/src/coverageBed/coverageMain.cpp index 7fb04ef2..81b6f230 100755 --- a/src/coverageBed/coverageMain.cpp +++ b/src/coverageBed/coverageMain.cpp @@ -103,6 +103,11 @@ void ShowHelp(void) { cerr << "Usage: " << PROGRAM_NAME << " [OPTIONS] -a <a.bed> -b <b.bed>" << endl << endl; + cerr << "Options: " << endl; + cerr << "\t-s\t" << "Force strandedness. That is, only include hits in A that" << endl; + cerr << "\t\toverlap B on the same strand." << endl; + cerr << "\t\t- By default, hits are included without respect to strand." << endl << endl; + cerr << "Output: " << endl; cerr << "\t" << " After each entry in B, reports: " << endl; cerr << "\t 1) The number of features in A that overlapped the B interval." << endl; diff --git a/src/coverageBed/new b/src/coverageBed/new new file mode 100644 index 00000000..e69de29b diff --git a/src/intersectBed/chr21.genes.bed b/src/intersectBed/chr21.genes.bed new file mode 100644 index 00000000..73d1706a --- /dev/null +++ b/src/intersectBed/chr21.genes.bed @@ -0,0 +1,828 @@ +chr21 9928613 10012791 uc002yip.1 24 - +chr21 9928613 10012791 uc002yiq.1 23 - +chr21 9928613 10012791 uc002yir.1 22 - +chr21 9928613 10012791 uc010gkv.1 19 - +chr21 9928613 10061300 uc002yis.1 33 - +chr21 10042683 10120808 uc002yiu.1 9 - +chr21 10042683 10120796 uc002yit.1 10 - +chr21 10079666 10120808 uc002yiv.1 4 - +chr21 10080031 10081687 uc002yiw.1 2 - +chr21 10081660 10120796 uc002yix.2 3 - +chr21 13332351 13346202 uc002yiy.2 5 + +chr21 13336975 13346202 uc002yiz.2 4 + +chr21 13361138 13412440 uc002yja.2 3 + +chr21 13904368 13935777 uc002yjb.1 11 + +chr21 13944438 13944477 uc002yjc.1 1 + +chr21 13945076 13945106 uc002yjd.1 1 + +chr21 13973491 13975330 uc002yje.1 1 - +chr21 14137333 14142556 uc002yjf.1 6 - +chr21 14200023 14200052 uc002yjg.1 1 + +chr21 14202070 14202096 uc002yjh.1 1 - +chr21 14237966 14274631 uc002yji.1 6 - +chr21 14270940 14274631 uc002yjj.2 2 - +chr21 14321612 14438647 uc002yjk.2 3 + +chr21 14321612 14438730 uc002yjl.2 3 + +chr21 14403005 14501125 uc002yjm.1 10 - +chr21 14459414 14483611 uc010gkw.1 4 - +chr21 14510336 14522564 uc002yjp.2 4 + +chr21 14510336 14522564 uc002yjn.2 5 + +chr21 14510336 14522564 uc002yjo.2 5 + +chr21 14567990 14585577 uc002yjq.1 5 + +chr21 14567990 14595563 uc002yjr.1 6 + +chr21 14567990 14595563 uc010gkx.1 5 + +chr21 14567990 14632206 uc002yjs.2 14 + +chr21 14665307 14677380 uc002yjt.1 5 - 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43658826 43671430 14 +uc002zdg.1 chr21 + 43694331 43698210 3 +uc002zdh.1 chr21 - 43706401 43722531 4 +uc002zdi.1 chr21 - 43773499 43903802 9 +uc002zdj.1 chr21 + 43809497 43809957 1 +uc002zdk.1 chr21 + 43903859 43940388 16 +uc002zdl.1 chr21 + 43930440 43940388 5 +uc002zdn.2 chr21 + 43963405 44006616 10 +uc010gpj.1 chr21 + 43963405 44006616 10 +uc002zdm.2 chr21 + 43963405 44006616 11 +uc002zdp.2 chr21 + 43973208 43983169 4 +uc002zdo.1 chr21 + 43973208 43983140 4 +uc002zdq.2 chr21 + 43985728 44006616 10 +uc002zdr.1 chr21 - 44018258 44020687 3 +uc002zds.2 chr21 + 44033845 44048411 13 +uc010gpk.1 chr21 + 44033845 44048411 12 +uc010gpl.1 chr21 + 44037239 44048411 10 +uc010gpm.1 chr21 + 44041669 44048411 8 +uc002zdt.1 chr21 - 44050068 44054670 4 +uc010gpn.1 chr21 - 44050068 44054670 2 +uc002zdu.1 chr21 - 44050068 44056876 2 +uc002zdv.1 chr21 + 44109543 44231903 10 +uc002zdw.1 chr21 + 44169706 44231903 9 +uc002zdx.1 chr21 + 44191238 44231903 11 +uc002zdy.1 chr21 + 44203942 44231903 9 +uc002zdz.2 chr21 + 44256633 44304536 7 +uc002zea.1 chr21 + 44256633 44350860 23 +uc010gpo.1 chr21 + 44256633 44350860 24 +uc002zeb.1 chr21 + 44351635 44375491 21 +uc002zed.2 chr21 + 44377921 44390033 6 +uc002zec.2 chr21 + 44377921 44390033 7 +uc002zee.1 chr21 - 44471149 44485262 7 +uc002zef.1 chr21 - 44471149 44485262 6 +uc010gpp.1 chr21 - 44473907 44485262 6 +uc002zeh.1 chr21 - 44490650 44506527 12 +uc002zeg.1 chr21 - 44490650 44506527 12 +uc002zei.1 chr21 + 44530190 44542530 14 +uc002zej.1 chr21 + 44534575 44542530 8 +uc010gpq.1 chr21 + 44534575 44542530 9 +uc010gpr.1 chr21 + 44534701 44542530 7 +uc002zek.1 chr21 + 44544357 44571684 25 +uc002zel.1 chr21 + 44544357 44571684 22 +uc002zen.1 chr21 + 44563661 44571684 10 +uc002zem.1 chr21 + 44563661 44571684 11 +uc002zer.2 chr21 - 44573254 44583713 7 +uc002zeq.2 chr21 - 44573254 44583713 7 +uc002zep.2 chr21 - 44573254 44583713 7 +uc002zeo.1 chr21 - 44573254 44583095 8 +uc010gps.1 chr21 - 44573254 44578835 4 +uc002zes.1 chr21 - 44578846 44583095 3 +uc002zet.1 chr21 + 44594473 44687392 33 +uc002zeu.1 chr21 + 44597566 44687392 32 +uc002zew.1 chr21 + 44597911 44687392 32 +uc010gpt.1 chr21 + 44597911 44687392 33 +uc002zex.1 chr21 + 44613431 44687392 28 +uc002zey.1 chr21 + 44635582 44687392 21 +uc002zez.1 chr21 - 44658905 44661051 2 +uc002zfa.1 chr21 + 44699820 44703167 2 +uc010gpu.1 chr21 + 44700955 44701729 1 +uc002zfb.1 chr21 - 44722649 44722699 1 +uc002zfc.1 chr21 - 44728608 44728644 1 +uc002zfd.1 chr21 - 44737584 44737655 1 +uc010gpv.1 chr21 - 44742202 44955923 13 +uc002zfe.1 chr21 - 44742202 44955923 12 +uc002zfg.2 chr21 + 44761525 44765140 2 +uc002zff.2 chr21 + 44761525 44765140 3 +uc002zfh.1 chr21 - 44783495 44784506 1 +uc002zfi.1 chr21 - 44794745 44795816 1 +uc002zfj.1 chr21 - 44802333 44803071 1 +uc002zfk.1 chr21 + 44818033 44819415 1 +uc002zfl.1 chr21 - 44823991 44824909 1 +uc002zfm.1 chr21 - 44835576 44836814 1 +uc002zfn.2 chr21 + 44844924 44846519 2 +uc002zfo.1 chr21 + 44856423 44857299 1 +uc002zfp.2 chr21 + 44871467 44872723 1 +uc002zfq.1 chr21 + 44881700 44882800 1 +uc002zfr.2 chr21 + 44890758 44891994 1 +uc002zfs.1 chr21 - 44898557 44899004 1 +uc002zft.1 chr21 + 44902276 44902686 1 +uc002zfu.1 chr21 - 44910533 44911272 1 +uc002zfv.1 chr21 - 44925918 44926506 1 +uc002zfw.1 chr21 + 44941514 44942387 1 +uc002zfy.1 chr21 - 45013382 45046166 6 +uc002zfx.1 chr21 - 45013382 45046166 7 +uc002zfz.1 chr21 - 45049959 45062472 4 +uc002zga.1 chr21 - 45049959 45062472 4 +uc002zgb.1 chr21 - 45093940 45118169 6 +uc002zgg.1 chr21 - 45130296 45173181 16 +uc010gpw.1 chr21 - 45130296 45165393 15 +uc002zgf.2 chr21 - 45130296 45165393 16 +uc002zge.1 chr21 - 45130296 45155785 16 +uc002zgd.1 chr21 - 45130296 45155169 15 +uc002zgh.2 chr21 + 45165393 45174016 4 +uc002zgi.1 chr21 - 45177156 45179316 2 +uc002zgk.2 chr21 - 45179070 45184188 3 +uc002zgj.2 chr21 - 45179070 45184188 4 +uc002zgl.1 chr21 + 45184382 45221316 6 +uc002zgm.1 chr21 + 45184382 45221316 6 +uc002zgn.1 chr21 - 45235614 45238429 2 +uc002zgo.2 chr21 - 45243556 45249070 2 +uc002zgu.1 chr21 + 45318942 45470902 12 +uc002zgw.1 chr21 + 45318942 45470902 12 +uc002zgv.1 chr21 + 45318942 45470902 12 +uc002zgt.1 chr21 + 45318942 45470902 11 +uc002zgs.1 chr21 + 45318942 45470902 13 +uc002zgr.1 chr21 + 45318942 45470902 13 +uc010gpx.1 chr21 + 45318942 45470902 11 +uc002zgq.1 chr21 + 45318942 45470902 13 +uc002zgp.1 chr21 + 45318942 45390638 10 +uc002zgy.1 chr21 + 45415952 45470902 10 +uc002zgz.1 chr21 + 45478694 45503073 3 +uc002zhd.1 chr21 - 45508270 45532239 8 +uc002zhc.1 chr21 - 45508270 45532239 9 +uc002zhb.1 chr21 - 45508270 45532239 10 +uc002zha.1 chr21 - 45508270 45521753 6 +uc002zhe.1 chr21 + 45532394 45536081 3 +uc002zhf.1 chr21 + 45539291 45541696 1 +uc002zhg.1 chr21 + 45649524 45758062 42 +uc002zhh.1 chr21 - 45664058 45669413 3 +uc002zhi.1 chr21 + 45699851 45758062 41 +uc010gpy.1 chr21 - 45742542 45788753 6 +uc002zhj.1 chr21 + 45748757 45758062 9 +uc002zhk.1 chr21 + 45751841 45758062 5 +uc002zhl.1 chr21 - 45759056 45786779 6 +uc010gpz.1 chr21 - 45759056 45786779 6 +uc002zhm.1 chr21 - 45759056 45786779 6 +uc002zhn.1 chr21 + 45837995 45841691 2 +uc010gqa.1 chr21 + 45877516 45878815 1 +uc010gqb.1 chr21 + 45888110 46180046 14 +uc002zho.1 chr21 - 46072302 46080761 3 +uc002zhp.1 chr21 + 46092504 46186795 14 +uc010gqc.1 chr21 + 46094302 46162867 8 +uc002zhq.1 chr21 + 46094302 46186795 14 +uc002zhr.1 chr21 + 46094302 46186795 14 +uc002zhs.1 chr21 + 46094302 46186795 13 +uc002zht.1 chr21 + 46140549 46186795 13 +uc002zhu.1 chr21 + 46226090 46249391 35 +uc010gqd.1 chr21 + 46242041 46249391 13 +uc002zhv.1 chr21 + 46245538 46249391 6 +uc002zhw.1 chr21 + 46246830 46249391 3 +uc002zhx.1 chr21 + 46281393 46283633 2 +uc002zib.1 chr21 + 46342460 46377191 9 +uc002zia.1 chr21 + 46342460 46377191 28 +uc002zhz.1 chr21 + 46342460 46374147 28 +uc002zhy.1 chr21 + 46342460 46374147 28 +uc002zic.1 chr21 + 46370831 46377191 2 +uc010gqe.1 chr21 + 46374170 46377189 1 +uc002zig.1 chr21 - 46380603 46399909 15 +uc002zif.1 chr21 - 46380603 46399909 15 +uc002zie.1 chr21 - 46380603 46387083 5 +uc002zih.1 chr21 - 46381121 46399909 14 +uc010gqf.1 chr21 - 46381121 46399909 13 +uc010gqg.1 chr21 - 46381326 46399909 14 +uc002zii.1 chr21 - 46405497 46428729 4 +uc002zij.1 chr21 - 46432787 46473119 23 +uc002zik.1 chr21 - 46433466 46473079 21 +uc002zil.1 chr21 - 46433466 46473119 22 +uc002zim.2 chr21 + 46473585 46496032 3 +uc002zin.2 chr21 + 46473585 46496032 4 +uc002zir.1 chr21 - 46479475 46529664 28 +uc002ziq.1 chr21 - 46479475 46511294 18 +uc002zip.1 chr21 - 46479475 46503607 13 +uc002zio.1 chr21 - 46479475 46491502 7 +uc002zis.1 chr21 + 46480565 46496171 3 +uc010gqh.1 chr21 + 46530694 46542093 4 +uc002ziy.1 chr21 + 46530694 46542093 4 +uc002zix.1 chr21 + 46530694 46542093 5 +uc002ziw.1 chr21 + 46530694 46542093 5 +uc002ziv.1 chr21 + 46530694 46542093 5 +uc002ziu.1 chr21 + 46530694 46536539 3 +uc002zit.1 chr21 + 46530694 46536539 3 +uc010gqi.1 chr21 + 46537349 46539139 2 +uc002zja.2 chr21 - 46544772 46565167 9 +uc002ziz.2 chr21 - 46544772 46562562 7 +uc002zjc.1 chr21 - 46545474 46563479 7 +uc002zjf.1 chr21 - 46545474 46568213 8 +uc010gqj.1 chr21 - 46545477 46568213 9 +uc002zjg.1 chr21 - 46559057 46568213 6 +uc002zji.2 chr21 + 46568463 46690110 47 +uc002zjj.1 chr21 + 46569229 46690110 47 +uc010gqk.1 chr21 + 46578738 46594162 6 +uc002zjk.1 chr21 - 46699327 46703021 2 +uc002zjl.1 chr21 + 46703317 46790647 20 +uc002zjm.1 chr21 + 46703317 46791548 21 +uc010gql.1 chr21 + 46703317 46791548 20 +uc002zjo.1 chr21 + 46703317 46813028 38 +uc002zjn.1 chr21 + 46703317 46794451 22 +uc002zjp.1 chr21 + 46748653 46789614 14 +uc002zjq.1 chr21 + 46783075 46791548 5 +uc002zjr.2 chr21 + 46797251 46803437 7 +uc002zjs.1 chr21 + 46805625 46813028 5 +uc002zjt.1 chr21 + 46808123 46813028 4 +uc002zjv.1 chr21 - 46842958 46849463 4 +uc002zju.1 chr21 - 46842958 46849463 3 +uc002zjw.2 chr21 + 46879954 46904483 7 +uc002zjx.1 chr21 + 46879954 46909291 12 +uc002zjy.1 chr21 + 46879954 46909291 11 +uc010gqm.1 chr21 + 46879954 46909291 9 +uc002zjz.1 chr21 + 46887625 46906276 6 diff --git a/src/intersectBed/intersectBed.cpp b/src/intersectBed/intersectBed.cpp index 7b4fde87..8971ff97 100755 --- a/src/intersectBed/intersectBed.cpp +++ b/src/intersectBed/intersectBed.cpp @@ -1,5 +1,5 @@ /***************************************************************************** - intersectBed.h + intersectBed.cpp (c) 2009 - Aaron Quinlan Hall Laboratory @@ -45,47 +45,61 @@ BedIntersect::~BedIntersect(void) { void BedIntersect::FindOverlaps(BED &a, vector<BED> &hits) { - // find all of the overlaps between a and B. - //bedB->binKeeperFind(bedB->bedMap[a.chrom], a.start, a.end, hits); - bedB->binKeeperFind(a.chrom, a.start, a.end, hits); - - int numOverlaps = 0; + // grab _all_ of the features in B that overlap with a. + bedB->FindOverlapsPerBin(a.chrom, a.start, a.end, a.strand, hits, this->forceStrand); + + // how many overlaps are there b/w a and B? + int numOverlaps = 0; - // should we print each overlap, or does the - // user want summary information? + // should we print each overlap, or does the user want summary information? bool printable = true; if (anyHit || noHit || writeCount) { printable = false; } - for (vector<BED>::const_iterator h = hits.begin(); h != hits.end(); ++h) { + // loop through the hits and report those that meet the user's criteria + vector<BED>::const_iterator h = hits.begin(); + vector<BED>::const_iterator hitsEnd = hits.end(); + for (; h != hitsEnd; ++h) { - // if forcing strandedness, move on if the hit - // is not on the same strand as A. - if ((this->forceStrand) && (a.strand != h->strand)) { - continue; // continue force the next iteration of the for loop. - } - else { - int s = max(a.start, h->start); - int e = min(a.end, h->end); + int s = max(a.start, h->start); + int e = min(a.end, h->end); + int overlapBases = (e - s); // the number of overlapping bases b/w a and b + int aLength = (a.end - a.start); // the length of a in b.p. + + // is there enough overlap relative to the user's request? (default ~ 1bp) + if ( ( (float) overlapBases / (float) aLength ) >= this->overlapFraction ) { + + // Report the hit if the user doesn't care about reciprocal overlap between A and B. + if (!reciprocal) { - // is there enough overlap relative to the user's request? - // (default ~ 1bp) - if ( ((float)(e-s) / (float)(a.end - a.start)) >= this->overlapFraction ) { + numOverlaps++; // we have another hit for A + if (!writeB && printable) { + if (writeA) bedA->reportBedNewLine(a); + else bedA->reportBedRangeNewLine(a,s,e); + } + else if (printable) { + if (writeA) { + bedA->reportBedTab(a); + bedB->reportBedNewLine(*h); + } + else { + bedA->reportBedRangeTab(a,s,e); + bedB->reportBedNewLine(*h); + } + } + } + else { // the user wants there to be sufficient reciprocal overlap + int bLength = (h->end - h->start); + float bOverlap = ( (float) overlapBases / (float) bLength ); - // Report the hit if the user doesn't care about reciprocal overlap - // between A and B. - if (!reciprocal) { + if (bOverlap >= this->overlapFraction) { numOverlaps++; // we have another hit for A if (!writeB && printable) { - if (writeA) { - bedA->reportBedNewLine(a); - } - else { - bedA->reportBedRangeNewLine(a,s,e); - } + if (writeA) bedA->reportBedNewLine(a); + else bedA->reportBedRangeNewLine(a,s,e); } else if (printable) { if (writeA) { @@ -98,34 +112,6 @@ void BedIntersect::FindOverlaps(BED &a, vector<BED> &hits) { } } } - else { - - float bOverlap = ((float)(e-s) / (float)(h->end - h->start)); - - if (bOverlap >= this->overlapFraction) { - - numOverlaps++; // we have another hit for A - - if (!writeB && printable) { - if (writeA) { - bedA->reportBedNewLine(a); - } - else { - bedA->reportBedRangeNewLine(a,s,e); - } - } - else if (printable) { - if (writeA) { - bedA->reportBedTab(a); - bedB->reportBedNewLine(*h); - } - else { - bedA->reportBedRangeTab(a,s,e); - bedB->reportBedNewLine(*h); - } - } - } - } } } } diff --git a/src/pairToBed/pairToBed.cpp b/src/pairToBed/pairToBed.cpp index 6913b8cc..f0317bd0 100755 --- a/src/pairToBed/pairToBed.cpp +++ b/src/pairToBed/pairToBed.cpp @@ -99,7 +99,7 @@ void BedIntersectPE::FindOverlaps(BEDPE &a, vector<BED> &hits1, vector<BED> &hit /* Find the quality hits between ***end1*** of the BEDPE and the B BED file */ - bedB->binKeeperFind(bedB->bedMap[a.chrom1], a.start1, a.end1, hits1); + bedB->binKeeperFind(a.chrom1, a.start1, a.end1, hits1); for (vector<BED>::iterator h = hits1.begin(); h != hits1.end(); ++h) { @@ -127,7 +127,7 @@ void BedIntersectPE::FindOverlaps(BEDPE &a, vector<BED> &hits1, vector<BED> &hit /* Now find the quality hits between ***end2*** of the BEDPE and the B BED file */ - bedB->binKeeperFind(bedB->bedMap[a.chrom2], a.start2, a.end2, hits2); + bedB->binKeeperFind(a.chrom2, a.start2, a.end2, hits2); for (vector<BED>::iterator h = hits2.begin(); h != hits2.end(); ++h) { @@ -218,7 +218,7 @@ void BedIntersectPE::FindSpanningOverlaps(BEDPE &a, vector<BED> &hits, string &t } spanLength = spanEnd - spanStart; - bedB->binKeeperFind(bedB->bedMap[a.chrom1], spanStart, spanEnd, hits); + bedB->binKeeperFind(a.chrom1, spanStart, spanEnd, hits); for (vector<BED>::iterator h = hits.begin(); h != hits.end(); ++h) { diff --git a/src/pairToPair/pairToPair.cpp b/src/pairToPair/pairToPair.cpp index c3fde140..1ef5a402 100755 --- a/src/pairToPair/pairToPair.cpp +++ b/src/pairToPair/pairToPair.cpp @@ -117,10 +117,10 @@ void PairToPair::FindOverlaps(BEDPE &a, vector<BED> &hitsA1B1, vector<BED> &hits // Find the _potential_ hits between each end of A and B - bedB->binKeeperFind(bedB->bedMapEnd1[a.chrom1], a.start1, a.end1, hitsA1B1); // hits between A1 to B1 - bedB->binKeeperFind(bedB->bedMapEnd1[a.chrom2], a.start2, a.end2, hitsA2B1); // hits between A2 to B1 - bedB->binKeeperFind(bedB->bedMapEnd2[a.chrom1], a.start1, a.end1, hitsA1B2); // hits between A1 to B2 - bedB->binKeeperFind(bedB->bedMapEnd2[a.chrom2], a.start2, a.end2, hitsA2B2); // hits between A2 to B2 + bedB->binKeeperFind(a.chrom1, a.start1, a.end1, hitsA1B1); // hits between A1 to B1 + bedB->binKeeperFind(a.chrom2, a.start2, a.end2, hitsA2B1); // hits between A2 to B1 + bedB->binKeeperFind(a.chrom1, a.start1, a.end1, hitsA1B2); // hits between A1 to B2 + bedB->binKeeperFind(a.chrom2, a.start2, a.end2, hitsA2B2); // hits between A2 to B2 // Now, reduce to the set of hits on each end of A and B that meet the required overlap fraction and orientation. diff --git a/src/shuffleBed/shuffleBed.cpp b/src/shuffleBed/shuffleBed.cpp index 91633b27..51ff1a29 100755 --- a/src/shuffleBed/shuffleBed.cpp +++ b/src/shuffleBed/shuffleBed.cpp @@ -145,7 +145,7 @@ void BedShuffle::ShuffleWithExclusions(istream &bedInput) { // test to see if the chosen locus overlaps // with an exclude region - exclude->binKeeperFind(exclude->bedMap[bedEntry.chrom], bedEntry.start, bedEntry.end, hits); + exclude->binKeeperFind(bedEntry.chrom, bedEntry.start, bedEntry.end, hits); bool haveOverlap = false; for (vector<BED>::const_iterator h = hits.begin(); h != hits.end(); ++h) { diff --git a/src/subtractBed/new b/src/subtractBed/new new file mode 100644 index 00000000..d2d55542 --- /dev/null +++ b/src/subtractBed/new @@ -0,0 +1 @@ +chr1 100 180 diff --git a/src/subtractBed/old b/src/subtractBed/old new file mode 100644 index 00000000..d2d55542 --- /dev/null +++ b/src/subtractBed/old @@ -0,0 +1 @@ +chr1 100 180 diff --git a/src/subtractBed/subtractBed.cpp b/src/subtractBed/subtractBed.cpp index d2ceff8b..8380428d 100755 --- a/src/subtractBed/subtractBed.cpp +++ b/src/subtractBed/subtractBed.cpp @@ -39,7 +39,7 @@ BedSubtract::~BedSubtract(void) { void BedSubtract::FindOverlaps(BED &a, vector<BED> &hits) { // find all of the overlaps between a and B. - bedB->binKeeperFind(bedB->bedMap[a.chrom], a.start, a.end, hits); + bedB->FindOverlapsPerBin(a.chrom, a.start, a.end, a.strand, hits, this->forceStrand); // is A completely spanned by an entry in B? // if so, A should not be reported. @@ -47,21 +47,19 @@ void BedSubtract::FindOverlaps(BED &a, vector<BED> &hits) { int numOverlaps = 0; vector<BED> bOverlaps; // list of hits in B. Special processing if there are multiple. - for (vector<BED>::iterator h = hits.begin(); h != hits.end(); ++h) { + vector<BED>::const_iterator h = hits.begin(); + vector<BED>::const_iterator hitsEnd = hits.end(); + for (; h != hitsEnd; ++h) { - // if forcing strandedness, move on if the hit - // is not on the same strand as A. - if ((this->forceStrand) && (a.strand != h->strand)) { - continue; // continue force the next iteration of the for loop. - } - int s = max(a.start, h->start); - int e = min(a.end, h->end); - + int e = min(a.end, h->end); + int overlapBases = (e - s); // the number of overlapping bases b/w a and b + int aLength = (a.end - a.start); // the length of a in b.p. + if (s < e) { // is there enough overlap (default ~ 1bp) - float overlap = ((float)(e-s) / (float)(a.end - a.start)); + float overlap = ((float) overlapBases / (float) aLength); if (overlap >= 1.0) { numOverlaps++; diff --git a/src/utils/bedFile/bedFile.cpp b/src/utils/bedFile/bedFile.cpp index 0cc6b2b5..bbec52f4 100755 --- a/src/utils/bedFile/bedFile.cpp +++ b/src/utils/bedFile/bedFile.cpp @@ -1,92 +1,29 @@ -// -// bedFile.cpp -// BEDTools -// -// Created by Aaron Quinlan Spring 2009. -// Copyright 2009 Aaron Quinlan. All rights reserved. -// -// Summary: Contains common functions for finding BED overlaps. -// -// Acknowledgments: Much of the code herein is taken from Jim Kent's -// BED processing code. I am grateful for his elegant -// genome binning algorithm and therefore use it extensively. +/***************************************************************************** + bedFile.cpp + (c) 2009 - Aaron Quinlan + Hall Laboratory + Department of Biochemistry and Molecular Genetics + University of Virginia + aaronquinlan@gmail.com + + Licensed under the GNU General Public License 2.0+ license. +******************************************************************************/ #include "lineFileUtilities.h" #include "bedFile.h" -// return the amount of overlap between two features. Negative if none. -int overlaps(const int aS, const int aE, const int bS, const int bE) { - return min(aE, bE) - max(aS, bS); -} - - -bool leftOf(const int a, const int b) { - return (a < b); -} - -// return the lesser of two values. -int min(const int a, int b) { - if (a <= b) { - return a; - } - else { - return b; - } -} - -// return the greater of two values. -int max(const int a, int b) { - if (a >= b) { - return a; - } - else { - return b; - } -} - - -int getBin(int start, int end) -/* - NOTE: Taken ~verbatim from kent source. - - Given start,end in chromosome coordinates assign it - * a bin. There's a bin for each 128k segment, for each - * 1M segment, for each 8M segment, for each 64M segment, - * and for each chromosome (which is assumed to be less than - * 512M.) A range goes into the smallest bin it will fit in. */ -{ - int startBin = start; - int endBin = end-1; - startBin >>= _binFirstShift; - endBin >>= _binFirstShift; - - for (int i=0; i<6; ++i) { - if (startBin == endBin) { - return binOffsetsExtended[i] + startBin; - } - startBin >>= _binNextShift; - endBin >>= _binNextShift; - } - - cerr << "start " << start << ", end " << end << " out of range in findBin (max is 512M)" << endl; - return 0; -} - -//********************************************* -// Sorting functions -//********************************************* -bool sortByChrom(BED const & a, BED const & b){ +bool sortByChrom(BED const & a, BED const & b) { if (a.chrom < b.chrom) return true; else return false; }; -bool sortByStart(const BED &a, const BED &b){ +bool sortByStart(const BED &a, const BED &b) { if (a.start < b.start) return true; else return false; }; -bool sortBySizeAsc(const BED &a, const BED &b){ +bool sortBySizeAsc(const BED &a, const BED &b) { unsigned int aLen = a.end - a.start; unsigned int bLen = b.end - b.start; @@ -95,7 +32,7 @@ bool sortBySizeAsc(const BED &a, const BED &b){ else return false; }; -bool sortBySizeDesc(const BED &a, const BED &b){ +bool sortBySizeDesc(const BED &a, const BED &b) { unsigned int aLen = a.end - a.start; unsigned int bLen = b.end - b.start; @@ -104,18 +41,18 @@ bool sortBySizeDesc(const BED &a, const BED &b){ else return false; }; -bool sortByScoreAsc(const BED &a, const BED &b){ +bool sortByScoreAsc(const BED &a, const BED &b) { if (a.score < b.score) return true; else return false; }; -bool sortByScoreDesc(const BED &a, const BED &b){ +bool sortByScoreDesc(const BED &a, const BED &b) { if (a.score > b.score) return true; else return false; }; -bool byChromThenStart(BED const & a, BED const & b){ +bool byChromThenStart(BED const & a, BED const & b) { if (a.chrom < b.chrom) return true; else if (a.chrom > b.chrom) return false; @@ -126,31 +63,64 @@ bool byChromThenStart(BED const & a, BED const & b){ return false; }; - -/* +int getBin(int start, int end) +/* NOTE: Taken ~verbatim from kent source. - Return a list of all items in binKeeper that intersect range. - Free this list with slFreeList. + Given start,end in chromosome coordinates assign it + * a bin. There's a bin for each 128k segment, for each + * 1M segment, for each 8M segment, for each 64M segment, + * and for each chromosome (which is assumed to be less than + * 512M.) A range goes into the smallest bin it will fit in. */ +{ + int startBin = start; + int endBin = end-1; + startBin >>= _binFirstShift; + endBin >>= _binFirstShift; + + for (int i=0; i<6; ++i) { + if (startBin == endBin) { + return binOffsetsExtended[i] + startBin; + } + startBin >>= _binNextShift; + endBin >>= _binNextShift; + } + + cerr << "start " << start << ", end " << end << " out of range in findBin (max is 512M)" << endl; + return 0; +} + +/* + Adapted from kent source "binKeeperFind" */ -void BedFile::binKeeperFind(string chrom, const int start, const int end, vector<BED> &hits) { +void BedFile::FindOverlapsPerBin(string chrom, int start, int end, string strand, vector<BED> &hits, bool forceStrand) { int startBin, endBin; - startBin = (start >>_binFirstShift); - endBin = ((end-1) >>_binFirstShift); + startBin = (start >> _binFirstShift); + endBin = ((end-1) >> _binFirstShift); + // loop through each bin "level" in the binning hierarchy for (int i = 0; i < 6; ++i) { + + // loop through each bin at this level of the hierarchy int offset = binOffsetsExtended[i]; - for (int j = (startBin+offset); j <= (endBin+offset); ++j) { + // loop through each feature in this chrom/bin and see if it overlaps + // with the feature that was passed in. if so, add the feature to + // the list of hits. vector<BED>::const_iterator bedItr = bedMap[chrom][j].begin(); vector<BED>::const_iterator bedEnd = bedMap[chrom][j].end(); - for (; bedItr != bedEnd; ++bedItr) { - if (overlaps(bedItr->start, bedItr->end, start, end) > 0) { - hits.push_back(*bedItr); - } + + // skip the hit if not on the same strand (and we care) + if (forceStrand && (strand != bedItr->strand)) { + continue; + } + else if (overlaps(bedItr->start, bedItr->end, start, end) > 0) { + hits.push_back(*bedItr); // it's a hit, add it. + } + } } startBin >>= _binNextShift; @@ -159,34 +129,40 @@ void BedFile::binKeeperFind(string chrom, const int start, const int end, vector } +void BedFile::countHits(const BED &a, bool forceStrand) { -void BedFile::countHits(map<int, vector<BED>, std::less<int> > &bk, BED &a, bool &forceStrand) { int startBin, endBin; - int i,j; + startBin = (a.start >> _binFirstShift); + endBin = ((a.end-1) >> _binFirstShift); - startBin = (a.start>>_binFirstShift); - endBin = ((a.end-1)>>_binFirstShift); - for (i=0; i<6; ++i) { + // loop through each bin "level" in the binning hierarchy + for (int i = 0; i < 6; ++i) { + + // loop through each bin at this level of the hierarchy int offset = binOffsetsExtended[i]; - - for (j = (startBin+offset); j <= (endBin+offset); ++j) { + for (int j = (startBin+offset); j <= (endBin+offset); ++j) { - for (vector<BED>::iterator el = bk[j].begin(); el != bk[j].end(); ++el) { + // loop through each feature in this chrom/bin and see if it overlaps + // with the feature that was passed in. if so, add the feature to + // the list of hits. + vector<BED>::iterator bedItr = bedMap[a.chrom][j].begin(); + vector<BED>::iterator bedEnd = bedMap[a.chrom][j].end(); + for (; bedItr != bedEnd; ++bedItr) { - if (forceStrand && (a.strand != el->strand)) { - continue; // continue force the next iteration of the for loop. + // skip the hit if not on the same strand (and we care) + if (forceStrand && (a.strand != bedItr->strand)) { + continue; } - else if (overlaps(el->start, el->end, a.start, a.end) > 0) { + else if (overlaps(bedItr->start, bedItr->end, a.start, a.end) > 0) { - el->count++; - el->depthMap[a.start+1].starts++; - el->depthMap[a.end].ends++; + bedItr->count++; + bedItr->depthMap[a.start+1].starts++; + bedItr->depthMap[a.end].ends++; - if (a.start < el->minOverlapStart) { - el->minOverlapStart = a.start; + if (a.start < bedItr->minOverlapStart) { + bedItr->minOverlapStart = a.start; } } - } } startBin >>= _binNextShift; diff --git a/src/utils/bedFile/bedFile.h b/src/utils/bedFile/bedFile.h index e9743f0b..327a1eec 100755 --- a/src/utils/bedFile/bedFile.h +++ b/src/utils/bedFile/bedFile.h @@ -1,3 +1,14 @@ +/***************************************************************************** + bedFile.h + + (c) 2009 - Aaron Quinlan + Hall Laboratory + Department of Biochemistry and Molecular Genetics + University of Virginia + aaronquinlan@gmail.com + + Licensed under the GNU General Public License 2.0+ license. +******************************************************************************/ #ifndef BEDFILE_H #define BEDFILE_H @@ -11,7 +22,7 @@ #include <algorithm> #include <limits.h> #include <cstdio> - +#include <tr1/unordered_map> using namespace std; //************************************************* @@ -58,23 +69,29 @@ struct BED { }; - -//************************************************* -// Common functions -//************************************************* - -int getBin(int, int); +// return the genome "bin" for a feature with this start and end +int getBin(int start, int end); -// BED sorting -bool sortByChrom(BED const &, BED const &); -bool sortByStart(const BED &, const BED &); -bool byChromThenStart(BED const &, BED const &); +// return the amount of overlap between two features. Negative if none and the the +// number of negative bases is the distance between the two. +inline +int overlaps(int aS, int aE, int bS, int bE) { + return min(aE, bE) - max(aS, bS); +} -// BED comparsions -int overlaps(const int, const int, const int, const int); -bool leftOf(const int, const int); -int min(const int, int); -int max(const int, int); +// return the lesser of two values. +inline +int min(int a, int b) { + if (a <= b) return a; + else return b; +} + +// return the greater of two values. +inline +int max(int a, int b) { + if (a >= b) return a; + else return b; +} // templated function to convert objects to strings @@ -88,20 +105,25 @@ std::string ToString(const T & value) // BED Sorting Methods -bool sortByChrom(BED const &, BED const &); -bool sortByStart(const BED &, const BED &); -bool sortBySizeAsc(const BED &, const BED &); -bool sortBySizeDesc(const BED &, const BED &); -bool sortByScoreAsc(const BED &, const BED &); -bool sortByScoreDesc(const BED &, const BED &); -bool byChromThenStart(BED const &, BED const &); +bool sortByChrom(const BED &a, const BED &b); +bool sortByStart(const BED &a, const BED &b); +bool sortBySizeAsc(const BED &a, const BED &b); +bool sortBySizeDesc(const BED &a, const BED &b); +bool sortByScoreAsc(const BED &a, const BED &b); +bool sortByScoreDesc(const BED &a, const BED &b); +bool byChromThenStart(BED const &a, BED const &b); + + //************************************************* // Common typedefs //************************************************* - typedef map<int, vector<BED>, std::less<int> > binsToBeds; +//typedef tr1::unordered_map<int, vector<BED> > binsToBeds; + typedef map<string, binsToBeds, std::less<string> > masterBedMap; +//typedef tr1::unordered_map<string, binsToBeds> masterBedMap; + typedef map<string, vector<BED>, std::less<string> > masterBedMapNoBin; @@ -118,36 +140,35 @@ public: // Destructor ~BedFile(void); - // parse an input line and determine how it should be handled - bool parseLine (BED &bed, const vector<string> &lineVector, int &lineNum); - // parse a BED line - bool parseBedLine (BED &bed, const vector<string> &lineVector, int lineNum); - // parse a GFF line - bool parseGffLine (BED &bed, const vector<string> &lineVector, int lineNum); - + // load a BED file into a map keyed by chrom, then bin. + // value is vector of BEDs void loadBedFileIntoMap(); + + // load a BED file into a map keyed by chrom + // value is vector of BEDs void loadBedFileIntoMapNoBin(); //void binKeeperFind(map<int, vector<BED>, std::less<int> > &, const int, const int, vector<BED> &); - void binKeeperFind(string chrom, const int start, const int end, vector<BED> &); - - - void countHits(map<int, vector<BED>, std::less<int> > &, BED &, bool &); + void FindOverlapsPerBin(string chrom, int start, int end, string strand, vector<BED> &hits, bool forceStrand); + //void countHits(map<int, vector<BED>, std::less<int> > &, BED &, bool &); + void countHits(const BED &a, bool forceStrand); + // printing methods void reportBedTab(const BED &); - void reportBedNewLine(const BED &); - + void reportBedNewLine(const BED &); void reportBedRangeTab(const BED &bed, int start, int end); void reportBedRangeNewLine(const BED &bed, int start, int end); - + // parse an input line and determine how it should be handled + bool parseLine (BED &bed, const vector<string> &lineVector, int &lineNum); + map<string, int> minPosMap; map<string, int> maxPosMap; // the bedfile with which this instance is associated string bedFile; - unsigned int bedType; // 3 -6 for BED + unsigned int bedType; // 3-6, 12 for BED // 9 for GFF vector<BED> bedVector; @@ -155,9 +176,11 @@ public: masterBedMapNoBin bedMapNoBin; private: - - + // parse a BED line + bool parseBedLine (BED &bed, const vector<string> &lineVector, int lineNum); + // parse a GFF line + bool parseGffLine (BED &bed, const vector<string> &lineVector, int lineNum); }; #endif /* BEDFILE_H */ diff --git a/src/utils/lineFileUtilities/lineFileUtilities.cpp b/src/utils/lineFileUtilities/lineFileUtilities.cpp index e99267fc..f21cbc6f 100755 --- a/src/utils/lineFileUtilities/lineFileUtilities.cpp +++ b/src/utils/lineFileUtilities/lineFileUtilities.cpp @@ -15,7 +15,7 @@ // Common Functions //*********************************************** -void Tokenize(const string& str, vector<string>& tokens) +void Tokenize(string str, vector<string>& tokens) { /* method to tokenize on any whitespace @@ -42,7 +42,6 @@ void Tokenize(const string& str, vector<string>& tokens) // Find next "non-delimiter" pos = str.find_first_of("\t", lastPos); } - } diff --git a/src/utils/lineFileUtilities/lineFileUtilities.h b/src/utils/lineFileUtilities/lineFileUtilities.h index 53080b22..e384dc39 100755 --- a/src/utils/lineFileUtilities/lineFileUtilities.h +++ b/src/utils/lineFileUtilities/lineFileUtilities.h @@ -9,7 +9,7 @@ using namespace std; // split a line from a file into a vector of strings. token = "\t" -void Tokenize(const string& str, vector<string>& tokens); +void Tokenize(string str, vector<string>& tokens); #endif /* LINEFILEUTILITIES_H */ diff --git a/src/windowBed/new b/src/windowBed/new new file mode 100644 index 00000000..e0d2dded --- /dev/null +++ b/src/windowBed/new @@ -0,0 +1,4 @@ +chr1 10000 20000 A.forward 1 + chr1 1000 8000 B1 +chr1 10000 20000 A.forward 1 + chr1 24000 32000 B2 +chr1 10000 20000 A.reverse 1 - chr1 1000 8000 B1 +chr1 10000 20000 A.reverse 1 - chr1 24000 32000 B2 diff --git a/src/windowBed/old b/src/windowBed/old new file mode 100644 index 00000000..e69de29b diff --git a/src/windowBed/windowBed.cpp b/src/windowBed/windowBed.cpp index 05d9ff44..d6d48b55 100755 --- a/src/windowBed/windowBed.cpp +++ b/src/windowBed/windowBed.cpp @@ -47,64 +47,60 @@ BedWindow::~BedWindow(void) { void BedWindow::FindWindowOverlaps(BED &a, vector<BED> &hits) { + /* + Adjust the start and end of a based on the requested window + */ + // update the current feature's start and end // according to the slop requested (slop = 0 by default) int aFudgeStart = 0; int aFudgeEnd; - // Does the user want to treat the windows based on strand? // If so, // if "+", then left is left and right is right // if "-", the left is right and right is left. if (this->strandWindows) { - if (a.strand == "+") { - if ((a.start - this->leftSlop) > 0) { - aFudgeStart = a.start - this->leftSlop; - } - else { - aFudgeStart = 0; - } + if ((a.start - this->leftSlop) > 0) aFudgeStart = a.start - this->leftSlop; + else aFudgeStart = 0; aFudgeEnd = a.end + this->rightSlop; } else { - if ((a.start - this->rightSlop) > 0) { - aFudgeStart = a.start - this->rightSlop; - } - else { - aFudgeStart = 0; - } + if ((a.start - this->rightSlop) > 0) aFudgeStart = a.start - this->rightSlop; + else aFudgeStart = 0; aFudgeEnd = a.end + this->leftSlop; } } + // If not, add the windows irrespective of strand else { - if ((a.start - this->leftSlop) > 0) { - aFudgeStart = a.start - this->leftSlop; - } - else { - aFudgeStart = 0; - } + if ((a.start - this->leftSlop) > 0) aFudgeStart = a.start - this->leftSlop; + else aFudgeStart = 0; aFudgeEnd = a.end + this->rightSlop; } - bedB->binKeeperFind(bedB->bedMap[a.chrom], aFudgeStart, aFudgeEnd, hits); + + /* + Now report the hits (if any) based on the window around a. + */ + // get the hits in B for the A feature + bedB->FindOverlapsPerBin(a.chrom, aFudgeStart, aFudgeEnd, a.strand, hits, this->matchOnStrand); int numOverlaps = 0; - for (vector<BED>::iterator h = hits.begin(); h != hits.end(); ++h) { - // if forcing strandedness, move on if the hit - // is not on the same strand as A. - if ((this->matchOnStrand) && (a.strand != h->strand)) { - continue; // continue force the next iteration of the for loop. - } + // loop through the hits and report those that meet the user's criteria + vector<BED>::const_iterator h = hits.begin(); + vector<BED>::const_iterator hitsEnd = hits.end(); + for (; h != hitsEnd; ++h) { int s = max(aFudgeStart, h->start); int e = min(aFudgeEnd, h->end); - + int overlapBases = (e - s); // the number of overlapping bases b/w a and b + int aLength = (a.end - a.start); // the length of a in b.p. + if (s < e) { // is there enough overlap (default ~ 1bp) - if ( ((float)(e-s) / (float)(a.end - a.start)) > 0 ) { + if ( ((float) overlapBases / (float) aLength) > 0 ) { numOverlaps++; if (!anyHit && !noHit && !writeCount) { bedA->reportBedTab(a); @@ -146,7 +142,7 @@ void BedWindow::WindowIntersectBed(istream &bedInput) { Tokenize(bedLine,bedFields); BED a; - // find the overlaps with B if it's a valid BED entry. + // find the overlaps between "a" and "B" if "a" is a valid BED entry. if (bedA->parseLine(a, bedFields, lineNum)) { FindWindowOverlaps(a, hits); hits.clear(); diff --git a/src/windowBed/windowMain.cpp b/src/windowBed/windowMain.cpp index dbe92896..2507bc58 100755 --- a/src/windowBed/windowMain.cpp +++ b/src/windowBed/windowMain.cpp @@ -191,7 +191,6 @@ void ShowHelp(void) { cerr << "\t\t- Default is 1000 bp." << endl; cerr << "\t\t- (INTEGER)" << endl << endl; - cerr << "\t-sw\t" << "Define -l and -r based on strand. For example if used, -l 500" << endl; cerr << "\t\tfor a negative-stranded feature will add 500 bp downstream." << endl; cerr << "\t\t- Default = disabled." << endl << endl; -- GitLab