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# ketterthill cold storage backup plan
To rescue Ketterthill’s reagents, security guard 5555 will ask you to come on site and give support to place their 8 shelves in our Cold storage room BT2 SS1-003A as illustrated on the drawing (Appendix 05). A person from Ketterthill will wait for you on the parking at -1st floor.
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# P5.1: Replacement of N2 or CO2 gas bottles (BT1/BT2)
# P5.1: Replacement of N2 or CO2 gas bottles (BT1/BT2)
This section describes the procedure for replacement of gas bottles.
If you receive an alarm on the gas, you need to come to LCSB.
If you receive an alarm on the gas, you need to come to LCSB.
1. A first method to identify which type of bottle is empty is to check on the gas panel in the corridor of the 4th floor in BT1 and room 104, 1st floor in BT2.
1. A first method to identify which type of bottle is empty is to check on the gas panel in the corridor of the 4th floor in BT1 and room 104, 1st floor in BT2.
<div align="center">
<img src="p5.1_img_01.jpg" height="200">
</div>
</div>
* If one LED is on, one couple of bottle is empty, only one couple of bottles needs to be replaced.
* If two LEDs are on (left and right), both couple of gas bottles are empty and need to be replaced urgently.
* If one LED is on, one couple of bottle is empty, only one couple of bottles needs to be replaced.
* If two LEDs are on (left and right), both couple of gas bottles are empty and need to be replaced urgently.
2. Press the reset button to stop the beeping.
2. Press the reset button to stop the beeping.
3. A second way to identify which type of gas and which couple of bottles is empty, is to directly check the manometers in the gas deposit rooms.
If the manometer (5 in the picture below) displays 0 bar, this means that the couple of bottles is empty.
If the manometer (5 in the picture below) displays 0 bar, this means that the couple of bottles is empty.
4. If you can see on the “Gas Bottle Exchange Sheet” in the room that the CO2 bottles have already been changed in the 48 h prior to the alarm, this is indicative of a pipeline infrastructure break down → see P6: gas network total failure back up plan.
4. If you can see on the “Gas Bottle Exchange Sheet” in the room that the CO2 bottles have already been changed in the 48 h prior to the alarm, this is indicative of a pipeline infrastructure break down → see P6: gas network total failure back up plan.
5. Replace the empty bottles in room 005 of BT1 or LT03 of BT2, ground floor
5. Replace the empty bottles in room 005 of BT1 or LT03 of BT2, ground floor
|*Personal protective equipment*|
|:-----------------------------:|
|*Personal protective equipment*|
|:-----------------------------:|
|Lab coat|
|Safety goggles|
<div align="center">
<img src="p5.1_img_02.jpg">
</div>
* Check the gas level on the manometer (5 on the picture). When empty, it displays 0 bar.
* Once the bottles that need to be changed have been identified, close the network valve (3 on the picture)
* Close the bottles (1 on the picture)
* Open the purge valve (4 on the picture) to release the pressure remaining in the pipe connecting the bottle, then close back the purge valve
* Unscrew the tubing from the bottle by hand (2 on the picture)
* Move the empty bottle to the bottle rack, bring the new bottle and fasten the chain
</div>
* Check the gas level on the manometer (5 on the picture). When empty, it displays 0 bar.
* Once the bottles that need to be changed have been identified, close the network valve (3 on the picture)
* Close the bottles (1 on the picture)
* Open the purge valve (4 on the picture) to release the pressure remaining in the pipe connecting the bottle, then close back the purge valve
* Unscrew the tubing from the bottle by hand (2 on the picture)
* Move the empty bottle to the bottle rack, bring the new bottle and fasten the chain
* Connect the new bottle by screwing the tubing hand tight (2 on the picture)
* Open the gas bottle (1 on the picture)
* Check for leaks at the connection between the bottle and the tubing
* Open the purge valve (4 on the picture) for 2 seconds to fill the tubing with gas and remove the air, then close it back
* Open the network valve (3 on the picture)
* Open the gas bottle (1 on the picture)
* Check for leaks at the connection between the bottle and the tubing
* Open the purge valve (4 on the picture) for 2 seconds to fill the tubing with gas and remove the air, then close it back
* Open the network valve (3 on the picture)
6. Check for any leak after connecting the bottle using the leak detector spray located in the gas deposit room, an example is shown on the picture below
6. Check for any leak after connecting the bottle using the leak detector spray located in the gas deposit room, an example is shown on the picture below
<div align="center">
<img src="p5.1_img_03.jpg" height="250">
</div>
7. Fill up the “gas bottle exchange record sheet” (Appendix 03) on the door inside the gas deposit room. A copy of this document is present in each gas deposit room
7. Fill up the “gas bottle exchange record sheet” (Appendix 03) on the door inside the gas deposit room. A copy of this document is present in each gas deposit room
<div align="center">
<img src="p5.1_img_04.jpg">
</div>
</div>
8. Check in the LIMS if the alert is resolved
8. Check in the LIMS if the alert is resolved
9. Notify the LCSB duty call manager and their backup by email, fill and fill the:
[Duty call report form](https://ulsurvey.uni.lu/index.php/936567?lang=en)
......
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# P5.2: CO2 Gas bottle replacement BTA
This section describes the procedure for replacement of gas bottles.
# P5.2: CO2 Gas bottle replacement BTA
This section describes the procedure for replacement of gas bottles.
1. Check when the bottle where exchanged for the last time on the “gas bottle exchange record sheet”.
1. Check when the bottle where exchanged for the last time on the “gas bottle exchange record sheet”.
* If the CO2 bottles have already been changed in the 48 h prior to the alarm, change the bottles and immediately contact the Facility Manager (LCSB-INF-01-A-01)
* If the last change was made more than 48h before, change the bottles and contact the duty call manager (see below)
* If the CO2 bottles have already been changed in the 48 h prior to the alarm, change the bottles and immediately contact the Facility Manager (LCSB-INF-01-A-01)
* If the last change was made more than 48h before, change the bottles and contact the duty call manager (see below)
2. Replace the empty bottles in room S130
2. Replace the empty bottles in room S130
<div align="center">
<img src="p.5.2_img-1.jpg">
</div>
* Check the gas level on the manometer (5 on the picture). When empty, it displays 0 bar.
* Once the pair of bottles that need to be changed have been identified, close the network valve (3 on the picture)
* Close the bottles (1 on the picture)
* Open the purge valve (4 on the picture) to release the pressure remaining in the pipe connecting the bottles, then close back the purge valve
* Unscrew the tubing from the bottles by hand (2 on the picture)
* Move the empty bottles to the bottle rack, bring the new bottles and fasten the chains
</div>
* Check the gas level on the manometer (5 on the picture). When empty, it displays 0 bar.
* Once the pair of bottles that need to be changed have been identified, close the network valve (3 on the picture)
* Close the bottles (1 on the picture)
* Open the purge valve (4 on the picture) to release the pressure remaining in the pipe connecting the bottles, then close back the purge valve
* Unscrew the tubing from the bottles by hand (2 on the picture)
* Move the empty bottles to the bottle rack, bring the new bottles and fasten the chains
* Connect the new bottles by screwing the tubing hand tight (2 on the picture)
* Open the gas bottles (1 on the picture)
* Check for leaks at the connection between the bottles and the tubing
* Open the purge valve (4 on the picture) for 2 seconds to fill the tubing with gas and remove the air, then close it back
* Open the network valve (3 on the picture)
* Check for any leak after connecting the bottle using the leak detector spray, an example is shown on the picture below
* Open the gas bottles (1 on the picture)
* Check for leaks at the connection between the bottles and the tubing
* Open the purge valve (4 on the picture) for 2 seconds to fill the tubing with gas and remove the air, then close it back
* Open the network valve (3 on the picture)
* Check for any leak after connecting the bottle using the leak detector spray, an example is shown on the picture below
<div align="center">
<img src="p.5.2_img-2.jpg" height="250">
</div>
4. Fill up the “gas bottle exchange record sheet” (LCSB-INF-01-A-03) on the door inside the gas deposit room.
A copy of this document is present in each gas deposit room.
A copy of this document is present in each gas deposit room.
<div align="center">
<img src="p.5.2_img-3.jpg">
</div>
</div>
1. Notify the Facility Manager, the LCSB duty call manager and their backup by email and fill the [Duty call report form](https://ulsurvey.uni.lu/index.php/936567?lang=en)
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# P5: Replacement of N2 or CO2 gas bottles (BT1/BT2)
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