Commit ad9381d7 authored by Laurent Heirendt's avatar Laurent Heirendt
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# P6: Gas network total failure backup plan BT1/BT2
## CO2
This process covers the actions to take in case of total failure of the carbon dioxide (CO2, dioxyde de carbone) gas network due to pipeline infrastructure breakdown.
1. Check the CO2 connection at the back of each incubator and at the connection of the manometer to identify a possible leak.
To do so, use the leak detector spray or the gas leak detector device.
2. Also investigate the LSRU floors
3. If a leak can be identified, fix it:
- plug the tubing back
- close the manometer connecting the incubator to the network (see arrow on the following picture): transfer the cells to another incubator
<div align="center">
<img src="p6_img_1.png">
</div>
4. If there no leak is detected, call 5555 and ask them to contact the SIL duty call.
5. Notify the LCSB duty call manager and their backup by email, fill and fill the [Duty call report form](https://ulsurvey.uni.lu/index.php/936567?lang=en)
## N2
This process covers the actions to take in case of total failure of the nitrogen (N2, azote) gas network due to pipeline infrastructure breakdown.
It consists in the rescue of glove boxes and hypoxic incubators by connecting them to bottles of nitrogen.
1. Identify the pipeline infrastructure breakdown:
- Check the “gas bottle exchange record sheet” (Appendix 03) located on the door inside the gas deposit room.
- If the N2 bottles have been changed in the last 48 h, this is indicative of a pipeline infrastructure break down.
2. Check the N2 connection at the back of each glovebox and hypoxic incubator and at the connection of the manometer to identify a possible leak.
To do so, use the leak detector spray or the gas leak detector device.
3. Also investigate the LSRU floors
4. If a leak can be identified, fix it:
- plug the tubing back
- close the manometer, do not transfer the samples
5. If there no leak is detected, call 5555 and ask them to contact the SIL duty call.
6. Notify the LCSB duty call manager and their backup by email, fill and fill the [Duty call report form](https://ulsurvey.uni.lu/index.php/936567?lang=en)
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# P7.2: Fridge or freezer failure BTA
This section describes the procedure in case of alarm on fridge or freezer.
1. Identify whether the issue comes from the **basement** or from one of the laboratories.
- If it comes from the basement, you can take action:
- Evaluate the origin of the alarm: door not properly closed, power failure, displaced probe…
- If the issue cannot be fixed,
- 4°C: transfer the content to the fridge in the same floor. Only one of the two fridges is under monitoring. If there is an issue with the temperature, transfer the content to the non-monitored fridge
- -80°C: if there is an issue with one of the compressor, the second is able to take over and to keep the temperature at -65°C. There is no need to move the samples.
- Keep track of what was moved where
- Before leaving, make sure that everything is back to normal or that the transitory situation is under control
- In case of issue, contact the facility manager
|*Personal protective equipment*|
|:-----------------------------:|
|Lab coat|
|Gloves|
- If it comes from any of the laboratories, contact the Mouse Facility Manager (see LCSB-INF-01 Appendix 01) and explain him the issue.
1. Notify the Facility Manager, the LCSB duty call manager and their backup by email and fill the [Duty call report form](https://ulsurvey.uni.lu/index.php/936567?lang=en)
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# P9.2: Intervention for emergency situations BTA
This procedure has to be applied in the event of any problem with the animal facility, major water leak, fire or ventilation issue.
1. Call the PC security (+352 46 66 44 5555) and explain the situation and ask them to contact the SIL duty call.
2. If necessary, request the intervention of public rescue (112 for firemen and ambulances; 113 for the police) and be available when they arrive on site.
3. Notify the Facility Manager, the LCSB duty call manager and their backup by email and fill the [Duty call report form](https://ulsurvey.uni.lu/index.php/936567?lang=en).
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