Commit ad9381d7 authored by Laurent Heirendt's avatar Laurent Heirendt
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move to dutycall

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# ketterthill cold storage backup plan
To rescue Ketterthill’s reagents, security guard 5555 will ask you to come on site and give support to place their 8 shelves in our Cold storage room BT2 SS1-003A as illustrated on the drawing (Appendix 05). A person from Ketterthill will wait for you on the parking at -1st floor.
Notify the LCSB duty call manager and their backup by email, fill the “Duty call report form” (https://ulsurvey.uni.lu/index.php/936567?lang=en)
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# P3: Incubator failure
This process does not concern LCSB0814 and LCSB1047 in BT2, 3rd floor, room 310 (See "P4: Zebrafish incubator temperature problem").
This process applies in case of problem with temperature or gas (N2 or CO2) levels of an incubator.
1. If after 20 minutes, the trend does not go back to the initial settings.
2. If you suspect an open door, you can ask a security guard to close it (+352 46 66 44 5555).
3. If it is not the problem, come on site to evaluate the situation.
4. If the issue cannot be fixed on site, transfer the samples to another incubator in the same room with the same temperarure and gas setting.
Personal protective equipment
-Lab coat
-Gloves
5. Keep track of what was moved where.
6. Notify the LCSB duty call manager and their back up by email, and fill the "Duty call report form" (https://ulsurvey.uni.lu/index.php/936567?lang=eng)
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# P5.1: Replacement of N2 or CO2 gas bottles (BT1/BT2)
This section describes the procedure for replacement of gas bottles.
If you receive an alarm on the gas, you need to come to LCSB.
1. A first method to identify which type of bottle is empty is to check on the gas panel in the corridor of the 4th floor in BT1 and room 104, 1st floor in BT2.
<div align="center">
<img src="p5.1_img_01.jpg" height="200">
</div>
* If one LED is on, one couple of bottle is empty, only one couple of bottles needs to be replaced.
* If two LEDs are on (left and right), both couple of gas bottles are empty and need to be replaced urgently.
2. Press the reset button to stop the beeping.
3. A second way to identify which type of gas and which couple of bottles is empty, is to directly check the manometers in the gas deposit rooms.
If the manometer (5 in the picture below) displays 0 bar, this means that the couple of bottles is empty.
4. If you can see on the “Gas Bottle Exchange Sheet” in the room that the CO2 bottles have already been changed in the 48 h prior to the alarm, this is indicative of a pipeline infrastructure break down → see P6: gas network total failure back up plan.
5. Replace the empty bottles in room 005 of BT1 or LT03 of BT2, ground floor
|*Personal protective equipment*|
|:-----------------------------:|
|Lab coat|
|Safety goggles|
<div align="center">
<img src="p5.1_img_02.jpg">
</div>
* Check the gas level on the manometer (5 on the picture). When empty, it displays 0 bar.
* Once the bottles that need to be changed have been identified, close the network valve (3 on the picture)
* Close the bottles (1 on the picture)
* Open the purge valve (4 on the picture) to release the pressure remaining in the pipe connecting the bottle, then close back the purge valve
* Unscrew the tubing from the bottle by hand (2 on the picture)
* Move the empty bottle to the bottle rack, bring the new bottle and fasten the chain
* Connect the new bottle by screwing the tubing hand tight (2 on the picture)
* Open the gas bottle (1 on the picture)
* Check for leaks at the connection between the bottle and the tubing
* Open the purge valve (4 on the picture) for 2 seconds to fill the tubing with gas and remove the air, then close it back
* Open the network valve (3 on the picture)
6. Check for any leak after connecting the bottle using the leak detector spray located in the gas deposit room, an example is shown on the picture below
<div align="center">
<img src="p5.1_img_03.jpg" height="250">
</div>
7. Fill up the “gas bottle exchange record sheet” (Appendix 03) on the door inside the gas deposit room. A copy of this document is present in each gas deposit room
<div align="center">
<img src="p5.1_img_04.jpg">
</div>
8. Check in the LIMS if the alert is resolved
9. Notify the LCSB duty call manager and their backup by email, fill and fill the:
[Duty call report form](https://ulsurvey.uni.lu/index.php/936567?lang=en)
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# P5.2: CO2 Gas bottle replacement BTA
This section describes the procedure for replacement of gas bottles.
1. Check when the bottle where exchanged for the last time on the “gas bottle exchange record sheet”.
* If the CO2 bottles have already been changed in the 48 h prior to the alarm, change the bottles and immediately contact the Facility Manager (LCSB-INF-01-A-01)
* If the last change was made more than 48h before, change the bottles and contact the duty call manager (see below)
2. Replace the empty bottles in room S130
<div align="center">
<img src="p.5.2_img-1.jpg">
</div>
* Check the gas level on the manometer (5 on the picture). When empty, it displays 0 bar.
* Once the pair of bottles that need to be changed have been identified, close the network valve (3 on the picture)
* Close the bottles (1 on the picture)
* Open the purge valve (4 on the picture) to release the pressure remaining in the pipe connecting the bottles, then close back the purge valve
* Unscrew the tubing from the bottles by hand (2 on the picture)
* Move the empty bottles to the bottle rack, bring the new bottles and fasten the chains
* Connect the new bottles by screwing the tubing hand tight (2 on the picture)
* Open the gas bottles (1 on the picture)
* Check for leaks at the connection between the bottles and the tubing
* Open the purge valve (4 on the picture) for 2 seconds to fill the tubing with gas and remove the air, then close it back
* Open the network valve (3 on the picture)
* Check for any leak after connecting the bottle using the leak detector spray, an example is shown on the picture below
<div align="center">
<img src="p.5.2_img-2.jpg" height="250">
</div>
4. Fill up the “gas bottle exchange record sheet” (LCSB-INF-01-A-03) on the door inside the gas deposit room.
A copy of this document is present in each gas deposit room.
<div align="center">
<img src="p.5.2_img-3.jpg">
</div>
1. Notify the Facility Manager, the LCSB duty call manager and their backup by email and fill the [Duty call report form](https://ulsurvey.uni.lu/index.php/936567?lang=en)
\ No newline at end of file
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# P8.1 Cryo storage BT2
This section describes the procedure in the event of low nitrogen levels in both the cryo storage and cryocyl-120 tanks (BT2, room SS1 003).
<div align="center">
<img src="p8.1_img-1.jpg" height="300">
</div>
1. If the issue is not the nitrogen level and the temperature cannot be held below -120°C, transfer the content of the cryostorage to the back up -80°C freezer LCSB0709 in the same room, see “P6: Freezer failure back up plan”.
|*Personal protective equipment*|
|:-----------------------------:|
|Lab coat|
|Cryogloves|
|Safety goggles|
2. If the issue comes from the nitrogen level, verify whether the cryocyl-120 tanks is empty by pressing on “press” on the level gauge panel: in that case, 0% is displayed
<div align="center">
<img src="p8.1_img-2.jpg" height="200">
</div>
3. If the cryocyl-120 is empty, it needs to be replaced by the liquid nitrogen tank standing in the same room.
* Unplug the black wire (power supply) of the right data logger (FW-Z 0,5) and secure the probe on the dedicated rack on the cryocyl-120 tank.
<div align="center">
<img src="p8.1_img-3.jpg">
</div>
4. Close gas valve number 1 on the cryocyl-120 tank.
<div align="center">
<img src="p8.1_img-4.jpg">
</div>
5. Unscrew the tubing connected to valve 1 using the wrench available in the duty call toolbox in the gas deposit rooms (Appendix 04)
6. Move the cryocyl-120 tank to the middle of the room
7. Unscrew the tubing connected to the liquid nitrogen tank and place the tank next to the cryo storage tank
<div align="center">
<img src="p8.1_img-5.jpg" height="300">
</div>
8. Screw the tubing from the cryo storage tank to the “Filling, withdrawal valve” valve (valve 1) of the liquid nitrogen tank using the wrench available in the duty call toolbox in the gas deposit rooms (Appendix 04)
9. Fully open valve 1, then close it back a ¼ turn
<div align="center">
<img src="p8.1_img-6.jpg">
</div>
10. Check that the liquid nitrogen percentage of the cryo storage tank increases (wait for 10 minutes)
11. Notify the LCSB duty call manager and their backup by email, fill and fill the [Duty call report form](https://ulsurvey.uni.lu/index.php/936567?lang=en)
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# P8.2: Cryostorage BTA
1. If you receive an alarm for low levels of liquid nitrogen, do not come on site.
2. The only case where you need to come is in the event of a temperature rise above - 80°C (however, if the nitrogen tank is empty, the temperature should be kept for a week.
There is no need to move the samples)
3. If this happens, transfer the samples to the -80°C freezer
4. Notify the Facility Manager, the LCSB duty call manager and their backup by email and fill the [Duty call report form](https://ulsurvey.uni.lu/index.php/936567?lang=en)
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# P3.2: Incubator failure
This process applies in the event of issues with temperature or gas levels of an incubator.
1. Contact the Animal facility manager (See LCSB-INF-01 Appendix 01) and explain him the situation
2. Notify the Facility Manager, the LCSB duty call manager and their backup by email and fill the [Duty call report form](https://ulsurvey.uni.lu/index.php/936567?lang=en)
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