diff --git a/README.md b/README.md index 7c52d2c780a184fc8ebb71cd573b84d57ad3f01f..b5a74e2578e76668123d11b4b1cdad78eb0f6b0f 100644 --- a/README.md +++ b/README.md @@ -1,25 +1,32 @@ # comparison-protocols -This project corresponds to the paper: A comparison of two iPSC differentiation protocols to generate human astrocyte cultures (Mulica et al) +This project corresponds to the paper: "A comparison of two iPSC differentiation protocols to generate human astrocyte cultures" (Mulica et al.) -#Figure2 -In this folder the codes for analyzing astrocytic morphology are stored, both for Oksanen and Palm astrocytes. +## Folder `Figure2` -Subfolder 'oksanen-astrocytes' contains the Matlab codes for analyzing the morphology of Oksanen astrocytes. The Matlab version: 2020a. The corresponding raw data is stored at: -/mnt/AtlasHCS/YokogawaCV8000Standalone/CellPathfinder/Patrycja/PKM_20210927_gfap_astro_OksanenMorph_20210927_205648/AssayPlate_PerkinElmer_CellCarrierUltra96 +In this folder, the codes for analyzing astrocytic morphology are stored, both for Oksanen and Palm astrocytes. + +Subfolder `oksanen-astrocytes` contains the Matlab codes for analyzing the morphology of Oksanen astrocytes. The Matlab version used was: 2020a. +The corresponding raw data is stored at: +``` +/mnt/AtlasHCS/YokogawaCV8000Standalone/CellPathfinder/Patrycja/PKM_20210927_gfap_astro_OksanenMorph_20210927_205648/AssayPlate_PerkinElmer_CellCarrierUltra96 /mnt/AtlasHCS/YokogawaCV8000Standalone/CellPathfinder/Patrycja/PKM_20210927_gfap_astro2_OksanenMorph2_20210927_222659/AssayPlate_PerkinElmer_CellCarrierUltra96 +``` -Subfolder 'palm-astrocytes'contains the Matlab codes for analyzing the morphology of Palm astrocytes. The Matlab version: 2020a. The corresponding raw data is stored at: +Subfolder `palm-astrocytes` contains the Matlab codes for analyzing the morphology of Palm astrocytes. The Matlab version used was: 2020a. The corresponding raw data is stored at: +``` /mnt/AtlasHCS/YokogawaCV8000Standalone/CellPathfinder/Patrycja/PKM_20210927_gfap_astro_PalmMorph_20211028_152159/AssayPlate_PerkinElmer_CellCarrierUltra96 +``` -Subfolder 'r-scripts' contain R scripts used to summarize the data. The graphs were generated using the summarized data in GraphPad. +Subfolder `r-scripts` contain R scripts used to summarize the data. The graphs were generated using the summarized data in GraphPad. -#Figure3, 4A, B5 +# Folder `Figure3, 4A, B5` The analysis was performed in R. -Raw data is stored: bds-02.uni.lux server in the "/mnt/data" +Raw data is stored at `bds-02.uni.lux` server in directory `/mnt/data` + +# Folder `Figure 4C, D, E and S3` -#Figure 4C, D, E and S3 The analysis was performed in R.