About

Synergistic effect of short- and long-read sequencing on functional meta-omics:

• assembly approaches: LR, SR and hybrid
• assembly comparison w.r.t.
  • basic stat.s (length, contiguity, coverage)
  • seq. similarity (incl. unique seq.s)
  • genomic reconstruction (plasmids, CRISPR, rRNA genes, AMR genes)
  • gene content (common/unique genes)
• GDB sample:
  • metaG and metaT coverage (contigs and genes)
  • functional characterization and peptide identification (w/ metaP)

Datasets

• zymo: mock community ("EVEN")
• nwc: natural whey starter culture, a low-complexity microbiome
• rumen: metagenomic sequencing of the cow rumen
• aquifer: groundwater (complex aquifer system)
• gdb: human fecal sample
  • from "generous donor B": metaG (Illuminar, ONT), metaT (Illumina), metaP
  • The origins of the study
    • original data prepared by Rashi (post-sequencing)
    • initial analyses performed by CCL

Files

• Manuscript
  • draft v1
• Raw data
• Processed data