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Last edited by Valentina Galata Nov 19, 2020
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Synergistic effect of short- and long-read sequencing on functional meta-omics:

  • assembly approaches: LR, SR and hybrid
  • assembly comparison w.r.t.
    • basic stat.s (length, contiguity, coverage)
    • seq. similarity (incl. unique seq.s)
    • genomic reconstruction (plasmids, CRISPR, rRNA genes, AMR genes)
    • gene content (common/unique genes)
  • GDB sample:
    • metaG and metaT coverage (contigs and genes)
    • functional characterization and peptide identification (w/ metaP)

Datasets

  • zymo: mock community ("EVEN")
  • nwc: natural whey starter culture, a low-complexity microbiome
  • rumen: metagenomic sequencing of the cow rumen
  • aquifer: groundwater (complex aquifer system)
  • gdb: human fecal sample
    • from "generous donor B": metaG (Illuminar, ONT), metaT (Illumina), metaP
    • The origins of the study
      • original data prepared by Rashi (post-sequencing)
      • initial analyses performed by CCL

Files

  • Manuscript
    • draft v1
  • Raw data
  • Processed data
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  • ONT_pilot_w_GDB_samples
  • Processed data
  • Raw data
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      • GDB.2019.rawdata.md5sums
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    • 576a5703db76fefda043f497f928130f
      • analysis.md5sums
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