Code used to generate metaP reports which are included in the report workflow. The reports were generated for - proteins from each assembly separately - all proteins from all assemblies together ```bash ##Generate parameters file java-cp SearchGUI-3.3.20.jar eu.isas.searchgui.cmd.IdentificationParametersCLI \ -out "params.par" \ -db "database.fasta" \ -prec_tol 10 \ -prec_ppm 1 \ -frag_tol 15 \ -frag_ppm 1 \ -digestion 0 \ -enzyme "Trypsin" \ -specificity 0 \ -mc 2 \ -fixed_mods "Carbamidomethylation of C" \ -variable_mods "Oxidation of M" \ -min_charge 2 \ -max_charge 4 \ -fi "b" \ -ri "y" \ -min_isotope 0 \ -max_isotope 1 \ -import_peptide_length_min 7 \ -import_peptide_length_max 60 \ -xtandem_noise_suppr 0 \ -xtandem_quick_acetyl 1 \ -xtandem_quick_pyro 1 \ -xtandem_refine 1 \ -msgf_instrument 1 \ -msgf_fragmentation 1 \ -msgf_protocol 0 \ -msgf_min_pep_length 7 \ -msgf_max_pep_length 60 \ -msgf_num_ptms 2 \ -comet_num_ptms 2 #SearchGUI search java -cp SearchGUI-3.3.20.jar eu.isas.searchgui.cmd.SearchCLI \ -spectrum_files "MGF_DIR" \ -output_folder "OUT_DIR" \ -id_params "params.par" \ -xtandem 1 \ -msgf 1 \ -comet 1 \ -andromeda 0 \ -output_default_name "Sample_Name" \ -output_option 0 \ -output_data 1 \ -output_date 1 #PeptideShaker analysis java -cp PeptideShaker-1.16.45.jar eu.isas.peptideshaker.cmd.PeptideShakerCLI \ -experiment "Sample_Name" \ -sample "1" \ -replicate "1" \ -identification_files "SearchGUI_Outpit" \ -out "Sample_Name.cpsx" \ -zip "Sample_Name.cpsx.zip" #PeptideShaker reports. java -cp PeptideShaker-1.16.45.jar eu.isas.peptideshaker.cmd.ReportCLI \ -in "Sample_Name.cpsx.zip" \ -out_reports "OUT_DIR" \ -reports 6,9 #Mzid Generation java -cp PeptideShaker-1.16.45.jar eu.isas.peptideshaker.cmd.MzidCLI \ -in "Sample_Name.cpsx.zip" \ -output_file "Sample_Name.mzid" \ -contact_first_name Benoit \ -contact_last_name Kunath \ -contact_email benoit.j.kunath@gmail.com \ -contact_address avenue de l’Université 7 Belval Luxembourg \ -organization_name LCSB \ -organization_email benoit.kunath@uni.lu \ -organization_address avenue de l’Université 7 Belval Luxembourg ```